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The reviewer is correct in their observation that stimulation frequency cannot be varied independently of all other parameters, including pulse width, total light delivery, and pulse train duration. In this experiment, we kept the duty cycle constant in order to mitigate the possible confounding factor of variations in total light delivery, which can lead to heating artifacts (Christie et al., 2013). We apologize if this was not clear to the reviewer, and have clarified this decision in the revised manuscript (Results; Figure 4B legend).

As a result of keeping the duty cycle constant, the pulse width associated with each frequency was necessarily different (30 ms for 10 Hz, 7.5 ms for 40 Hz, and 3 ms for 100 Hz). The alternative option – keeping the pulse width fixed and varying the pulse train duration to maintain total light delivery – would lead to uninterpretable results due to differences in neuronal adaptation that occur over the course of a 20 s pulse train compared to a 2 s pulse train (Note that a 10-fold difference would be required for comparing 10 and 100 Hz).

To address this concern as best as possible, we have performed additional fMRI experiments keeping both the pulse width and train duration constant. Our results show that both the increase in forebrain activation with frequency and the transition from negative to positive BOLD signals in cortex is preserved ( Figure 2—figure supplement 3 ). Although these stimulus trains deliver different amounts of total light, the fact that we see these phenomena while varying frequency and keeping either the total amount of light delivery ( Figures 2 and 3 ) or pulse width ( Figure 2—figure supplement 3 ) constant suggests that frequency is the main factor in driving these effects.

To address this concern, we have added a supplementary figure, which shows that the pre-stimulus forebrain EEG activity was consistent across frequencies of stimulation ( Figure 6—figure supplement 1 ). Thus, the differences in evoked behavior and electrographic responses can be attributed to the stimulation paradigm itself.

With regards to the decrease in cortical firing, the magnitude of change during 10 Hz stimulation was well correlated with the pre-stimulus firing rate (n = 46 neurons; R 2 = 0.78, see Author response image 1 ). This correlation is consistent with expectations, since cells which have a high baseline firing rate can be suppressed more than those with a low baseline firing rate. The data also show that cortical cells exhibiting a wide range of baseline firing rate were inhibited, emphasizing the robustness of central thalamus-driven cortical inhibition. To generate this plot, the average firing rate over the 20 s period before stimulation was compared with the average firing rate during the 5-10 s interval after stimulation began (that is, when the most neurons were silenced; see Table 1 ).

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content of the spleen was examined by the method of
Schneider (1957) . DNA and RNA were determined using diphenylamine and orcinol , respectively.

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A. hydrophila was inoculated in tryptone soya broth and incubated overnight in a shaker for 12h at 28°C. The culture was centrifuged at 800 g for 15min at 4°C. The packed cells were washed three times and then the required dose was prepared in phosphate buffered saline (pH 7.2). The bacterial suspension was prepared to 1×10 6 CFU as determined using a Neubauer haemocytometer. The stock cultures in tryptone soya broth (Himedia) were stored at −70°C with 0.85% (w/v) NaCl and 20% (v/v) to provide stable inocula throughout the study ( Yadav et al., 1992 ).

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Fishes were fed with 0%, 0.05%, 0.5% and 5% (later it will be called as control, group A, group B and group C, respectively) extract of supplemented diet at the rate of 2% of body weight per day for 60days.

Fishes were randomly divided into four groups (control, group A, group B and group C) in triplicate each with 12 fishes ( n =12 fishes/group) and were fed with experimental diet for 15days. They were challenged with heat killed A. hydrophila (1×10 6 cells/fish) through intraperitoneal (i.p.) injection. Then, they were fed with the same doses of diet for 7, 14, 21 and 28days. Total number of fish used in this assay is 144. After the end of every seven days, blood was collected from the fish for assaying specific immunological parameter. Simultaneously, the post-immunized fish spleen of all experimental groups was isolated for histological studies.

The fishes were bled at regular intervals of 7days post immunization to 28days. Antibody was produced against heat killed A. hydrophila , which were detected using an indirect ELISA according to Binuramesh et al. (2005) .

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. Six fishes per group were injected i.p. with 0.1ml of 5% SRBC after 28days of confinement for evaluating the effect of confinement in all experimental diet groups and control.

After immunization with A. hydrophila , the experimental fish spleen samples were taken at 7th day and 28th day of feeding trial. Spleen samples were removed and fixed in 10% neutral buffered formalin, embedded in paraffin and stained with Robert Clergerie Clergerie Paris TStrap Platform Sandals Cheap Manchester Great Sale Outlet New TwRtwZAnM
and eosin (HE) for optical examination (
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). Micrograph of spleen was taken from the paraffin sections at a final magnification of 400 using a Nikon Microphot-FXA microscope and an Olympus DP50 camera. For each group, micrographs originating from six fishes ( n =12) were analysed.

Pros

Big display, looks great, price is fantastic and this is regarded by many owners as the best Android Wear watch out there.

Cons

Display might be a little too big and battery life is a problem (common with all smartwatches except those made by Pebble, really).

How we decided

We purchase every product we review with our own funds — we never accept anything from product manufacturers.

Zero products received from manufacturers.
Why trust BestReviews?
BestReviews spends thousands of hours researching, analyzing, and testing products to recommend the best picks for most consumers. We buy all products with our own funds, and we never accept free products from manufacturers.
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Last Updated July 2018

Back in 1970, the Hamilton Watch Company teamed up with an emerging tech company to create the first digital watch — a timepiece that was considered pretty “smart” because it flashed the date and time on a small LED screen.

Fast forward 30+ years, and such an innovation seems like ancient history. But that first digital watch laid the groundwork for today’s smartwatch category.

While the term “smartwatch” defies a precise definition, you can think of it as a wearable computer that sits on your wrist. Microsoft had such a concept in mind when it introduced the Spot in 2003, but the company discontinued the idea. It took more than a decade (with a few stops in between from companies such as Sony, Samsung, and Motorola) for leading consumer tech giant Apple to release its first version of the smartwatch.

The evolution of the smartwatch continues today. Manufacturers of these wearable devices have yet to zero in on exactly which features and functions — not to mention price points — meet the demands of tech-hungry consumers.

Still, you can’t deny the appeal of the smartwatch, and if you’ve landed on this page, chances are you’re interested in one for yourself or a loved one.

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Most smartwatches nowadays come with sharing features that can help you track your movements throughout the day, or even compete with others for fitness goals.

Types of smartwatches

While smartwatches are small, wearable computers that hug your wrist, that’s where the similarities between product options end. The smartwatch space spans many product types and can be divided into a few standard categories.

Fitness smartwatches

From such manufacturers as Fitbit (which recently purchased Pebble), Garmin, Microsoft, and Tomtom, these wearables are smaller in size than traditional smartwatches. The primary function of a fitness band is to provide support and tracking related to an individual’s exercise routine.

Price ranges from around $80 to over $200 and varies based on the watch’s capabilities, which can include heart rate monitoring and the measurement of calories burned and steps taken. Watches on the higher end of the pricing spectrum will feature notification of phone calls or texts but not the ability to answer calls or respond to messages.

To add GPS to a fitness band — a great asset for runners — you’re looking at a watch like the FitBit Surge, which sells for over $200 . However, the FitBit Blaze is extremely popular with consumers and sells for $189. Though GPS isn’t included in the band itself, you can sync it with your smartphone for connected GPS service.

Notification smartwatches

Primarily manufactured by traditional watchmakers such as Timex and Fossil, these timepieces sync with a smartphone and provide alerts when a phone call comes in, when a social media message is posted, or when a text arrives. However, these gadgets lack the ability to respond to said notifications.

As the price increases in this category, you’ll find more complex devices that are powered by either the Apple or Android operating systems to facilitate advanced functionality.

Entry-level models offer basic fitness and sleep tracking. More advanced units may include a customizable face. In addition to syncing with an Android smartphone, the Fossil Q line has built-in WiFi and Bluetooth, providing users the ability to remotely control music as well as use voice to control its features.

Smartphone smartwatch companions

This is far and away the biggest category. Here you will find Apple’s smartwatch as well as models from Samsung, Motorola, LG, and most popular consumer electronics manufacturers.

All devices within this category use the manufacturer’s operating system, and each operating system and device is linked to a marketplace of applications that are exclusive to that device. For example, Samsung uses the Tizen operating system and Google usesthe Android operating system. Because of this, their applications are not, for the most part, interchangeable.

Smartwatches in this category operate in sync with their companion smartphones via WiFi and/or Bluetooth. For example, an Apple watch is tied to the iPhone, and the Samsung Gear smartwatch links with Samsung’s various phones. The phone must be in proximity of the watch in order for all applications to function properly.

Because this category of smartwatches does not include 3G or 4G LTE chipsets, they cannot independently make or receive phone calls.

Standalone smartwatches

For those who want their smartwatch to be able to receive or send phone calls independent of their smartphone, a number of manufacturers are beginning to add 3G or 4G LTE chips to their wrist wearables. Most notable in this category is the Samsung Gear S3, which has cell capability built in and works with ATT or Verizon.

Other smartwatches with LTE functionality include the LG Urbane and a number of products from smaller Asian manufacturers, the capabilities of which can be expanded into replicating a smartphone by using an eSim card.

On the horizon for 2017 are the long-anticipated smartwatches from Google-code named Angelfish and Swordfish, with the Swordfish including LTE (4G) functionality.

DID YOU KNOW?

It might sound pretty basic but, if you use your smartwatch more for fitness tracking, it is an important factor to pick which hand you want it to be on. Your dominant hand will be much more in use throughout the day than your other hand.

Smartwatch benefits

For those on the fence about buying a smartwatch, here are some benefits to consider:

A smartwatch helps you stay connected.

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